Journal: Molecular Medicine Reports

Article Title: Eph/ephrin signalling serves a bidirectional role in lipopolysaccharide-induced intestinal injury

doi: 10.3892/mmr.2018.9169

Figure Lengend Snippet: Effects of LPS on the expression of EphA2-Ephrin A1 and EphB1-Ephrin B3 signaling in vivo . (A) Western blot analysis of EphA2, Ephrin A1, Ephrin B3 and EphB1 expression in colon tissues. (B) Densitometry was performed to quantify the levels of the proteins. The results are expressed as the mean ± standard deviation (n=6/group). **P<0.01 vs. vehicle (PBS) group. Eph, erythropoietin-producing hepatocellular; LPS, lipopolysaccharide.

Article Snippet: To establish ENS cells overexpressing Ephrin B3, ENS cells were transfected with Ephrin B3 lentiviral activation particles (Santa Cruz Biotechnology, Inc., Dallas, TX, USA) in complete medium with Polybrene (5 μg/ml) after reaching 60% confluency and incubated overnight.

Techniques: Expressing, In Vivo, Western Blot, Standard Deviation

Journal: Molecular Medicine Reports

Article Title: Eph/ephrin signalling serves a bidirectional role in lipopolysaccharide-induced intestinal injury

doi: 10.3892/mmr.2018.9169

Figure Lengend Snippet: Effects of EphA2 antagonism on EphB1-Ephrin B3 expression in vivo . (A) Western blot analysis of EphA2, Ephrin A1, Ephrin B3 and EphB1 expression in the colon. (B) Densitometry was performed to quantify the levels of the proteins. The results are expressed as the mean ± standard deviation (n=6/group). ## P<0.01 vs. PBS+IgG (control); **P<0.01 vs. LPS+IgG treatment. Eph, erythropoietin-producing hepatocellular; LPS, lipopolysaccharide; mAb, monoclonal antibody; IgG, immunoglobulin G.

Article Snippet: To establish ENS cells overexpressing Ephrin B3, ENS cells were transfected with Ephrin B3 lentiviral activation particles (Santa Cruz Biotechnology, Inc., Dallas, TX, USA) in complete medium with Polybrene (5 μg/ml) after reaching 60% confluency and incubated overnight.

Techniques: Expressing, In Vivo, Western Blot, Standard Deviation, Control

Journal: Molecular Medicine Reports

Article Title: Eph/ephrin signalling serves a bidirectional role in lipopolysaccharide-induced intestinal injury

doi: 10.3892/mmr.2018.9169

Figure Lengend Snippet: Effects of Ephrin B3 overexpression on EphA2-Ephrin A1 signaling in vitro . (A) Western blotting and densitometry analysis of EphA2, Ephrin A1, Ephrin B3 and EphB1 expression. (B) Effect of Ephrin B3 overexpression on LPS-induced injury in primary cultured enteric neuronal and glial cells using the Cell Counting Kit-8 assay. (C) Effect of Ephrin B3 overexpression on LPS-induced injury in primary cultured enteric neuronal and glial cells using the LDH leakage assay. The results are expressed as the mean ± standard deviation (n=3). ## P<0.01 vs. control; **P<0.01 vs. LPS treatment. Eph, erythropoietin-producing hepatocellular; LPS, lipopolysaccharide; LDH, lactate dehydrogenase.

Article Snippet: To establish ENS cells overexpressing Ephrin B3, ENS cells were transfected with Ephrin B3 lentiviral activation particles (Santa Cruz Biotechnology, Inc., Dallas, TX, USA) in complete medium with Polybrene (5 μg/ml) after reaching 60% confluency and incubated overnight.

Techniques: Over Expression, In Vitro, Western Blot, Expressing, Cell Culture, Cell Counting, Standard Deviation, Control

Journal: Molecular Medicine Reports

Article Title: Eph/ephrin signalling serves a bidirectional role in lipopolysaccharide-induced intestinal injury

doi: 10.3892/mmr.2018.9169

Figure Lengend Snippet: Effects of Ephrin B3 overexpression on inflammatory cytokines in vitro . The levels of inflammatory cytokines in primary cultured enteric neuronal and glial cells were determined by ELISA. The results are expressed as the mean ± standard deviation (n=3). ## P<0.01 vs. control; **P<0.01 vs. LPS treatment. Eph, erythropoietin-producing hepatocellular; LPS, lipopolysaccharide; MCP-1, monocyte chemoattractant protein-1; TNF-α, tumour necrosis factor α; IL, interleukin; ICAM-1, intercellular adhesion molecule 1; VCAM-1, vascular cell adhesion molecule-1.

Article Snippet: To establish ENS cells overexpressing Ephrin B3, ENS cells were transfected with Ephrin B3 lentiviral activation particles (Santa Cruz Biotechnology, Inc., Dallas, TX, USA) in complete medium with Polybrene (5 μg/ml) after reaching 60% confluency and incubated overnight.

Techniques: Over Expression, In Vitro, Cell Culture, Enzyme-linked Immunosorbent Assay, Standard Deviation, Control

Journal: Molecular Medicine Reports

Article Title: Eph/ephrin signalling serves a bidirectional role in lipopolysaccharide-induced intestinal injury

doi: 10.3892/mmr.2018.9169

Figure Lengend Snippet: Effects of Ephrin B3 overexpression on Akt-NF-κB, Src-NF-κB and Wnt/β-catenin signalling in vitro . (A) Western blotting and densitometry analysis of the total expression and phosphorylation of Akt, Src and NF-κB p65. (B) Western blotting and densitometry analysis of the expression of E-cadherin and nuclear translocation of β-catenin. The results are expressed as the mean ± standard deviation (n=3). ## P<0.01 vs. control; **P<0.01 vs. LPS treatment. Eph, erythropoietin-producing hepatocellular; LPS, lipopolysaccharide; Akt, protein kinase B; NF-κB, nuclear factor-κB; p-, phosphorylated.

Article Snippet: To establish ENS cells overexpressing Ephrin B3, ENS cells were transfected with Ephrin B3 lentiviral activation particles (Santa Cruz Biotechnology, Inc., Dallas, TX, USA) in complete medium with Polybrene (5 μg/ml) after reaching 60% confluency and incubated overnight.

Techniques: Over Expression, In Vitro, Western Blot, Expressing, Phospho-proteomics, Translocation Assay, Standard Deviation, Control